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overexpression plasmid pbabe-puro  (Addgene inc)


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    Addgene inc overexpression plasmid pbabe-puro
    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
    Overexpression Plasmid Pbabe Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "NRF2 regulates lipid droplet dynamics to prevent lipotoxicity"

    Article Title: NRF2 regulates lipid droplet dynamics to prevent lipotoxicity

    Journal: iScience

    doi: 10.1016/j.isci.2025.112925

    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in sgNRF2/mG0S2-Flag-overexpression HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
    Figure Legend Snippet: The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in sgNRF2/mG0S2-Flag-overexpression HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.

    Techniques Used: Expressing, Control, One-tailed Test, Over Expression



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    overexpression plasmid pbabe-puro  (Addgene inc)
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    Addgene inc overexpression plasmid pbabe-puro
    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
    Overexpression Plasmid Pbabe Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    pbabe-puro  (Addgene inc)
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    pbabe cdkn1a p21 mutants pbabe purocdkn1a p21  (Addgene inc)
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in <t>sgNRF2/mG0S2-Flag-overexpression</t> HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.
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    The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in sgNRF2/mG0S2-Flag-overexpression HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.

    Journal: iScience

    Article Title: NRF2 regulates lipid droplet dynamics to prevent lipotoxicity

    doi: 10.1016/j.isci.2025.112925

    Figure Lengend Snippet: The negative regulator of adipose triglyceride lipase, G0S2, acts downstream of NRF2 to regulate LD abundance in HEK-TtH cells (A) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of regulators of LD structure, lipids, and lipid storage in sgKEAP1 cells vs. sgScr cells. Vertical dotted line represents no change relative to sgScr control mRNA levels. n = 3 biological replicates. Mean ± SD. ( p < 0.05 = ∗, p < 0.01 = ∗∗). (B) Fold change of mRNA expression (2-ΔΔCT relative to GAPDH) of G0S2 in lung cancer cell lines. Data represented normalized to G0S2 expression of H1650 cells. Horizontal dotted line represents no change relative to H1650 mRNA levels. n = 3 biological replicates. Mean ± SD. (C) Representative images of LDs in sgKEAP1/sgG0S2 double-KO HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (D) Quantification of LD area per cell from (C). n = 5 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD. (E) Representative images of LDs in sgNRF2/mG0S2-Flag-overexpression HEK-TtH cells taken with 63× objective. Scale bars represent 20 μm. (F) Quantification of LD area per cell from (E). n = 3 biological replicates. Statistics represented calculated by Student’s t test (one-tailed, unpaired with equal variance). Mean ± SD.

    Article Snippet: Overexpression plasmid pBabe-Puro (addgene #1764) was cut by multiple restriction enzymes, generating incompatible ends.

    Techniques: Expressing, Control, One-tailed Test, Over Expression